Kisspeptin Dosing: 125mcg Daily Before Bed

Kisspeptin is a neuropeptide that acts as the master upstream regulator of the reproductive hormone axis, triggering GnRH release which drives LH and FSH secretion. No kisspeptin formulation is FDA-approved. This is not medical advice.

Quick Reference: Standard Protocol

For the full kisspeptin peptide profile and vendor pricing, see our kisspeptin peptide page. For the science behind kisspeptin's mechanism, see our kisspeptin benefits guide.

Cycling Details

The standard protocol is 30 days on / 30 days off. This prevents KISS1R receptor desensitization — continuous kisspeptin exposure causes the receptor to downregulate within hours, blunting LH/FSH output (Dhillo et al., 2005).

Discrete daily injections (rather than continuous infusion) mimic natural pulsatility, since kisspeptin neurons fire in synchronized bursts roughly every 60-90 minutes. The short half-life of kisspeptin-10 (~4 minutes) means the receptor "resets" between daily doses.

Enhanced Protocol (Community)

For kisspeptin-10 users seeking more aggressive stimulation:

Kisspeptin-54 has a longer action window (~4-6 hours of LH elevation), so once daily dosing is typically sufficient. Weight-based clinical dosing: 3.2 nmol/kg SC (~21 mcg/kg for a 70 kg person = ~1,470 mcg).

Routes of Administration

Subcutaneous (standard): Lower abdomen or upper outer thigh. Rotate sites to prevent lipodystrophy. Use 29-31 gauge insulin syringe. No food timing requirements since it's injected subcutaneously.

IV (research only): Used in clinical studies for acute LH pulse characterization. Not practical for community use.

Reconstitution Quick Reference

Math: 5,000 mcg / 2 mL = 2,500 mcg/mL. 125 mcg / 2,500 = 0.05 mL = 5 units. Swirl gently, refrigerate, use within 28 days.

For step-by-step reconstitution instructions, see the BPC-157 reconstitution guide — same technique applies to all lyophilized peptides.

Where These Numbers Come From

Clinical studies where continuous kisspeptin-54 infusion initially spiked LH but then suppressed it after 6-12 hours established the critical importance of pulsatile dosing (Dhillo et al., 2005).

In IVF protocols, kisspeptin-54 has been studied as an alternative oocyte maturation trigger to hCG. A single subcutaneous dose of 12.8 nmol/kg triggered maturation with zero OHSS cases (Abbara et al., 2015; Jayasena et al., 2014).

The 2017 fMRI study found no adverse effects from kisspeptin-54 infusion in healthy men (Comninos et al., 2017), and the 2023 HSDD trial found kisspeptin well-tolerated with no treatment-related serious adverse events (Ertl et al., 2023).

Stacking Protocols

Kisspeptin occupies a unique position as an upstream hormonal regulator. It works through GnRH/LH pathways (hormonal) while PT-141 activates MC4R directly (central arousal) — complementary but unstudied together. For a detailed comparison, see our kisspeptin vs PT-141 comparison.

Side Effects & Safety

• Flushing/warmth — brief, reported in some subjects
• Injection site reactions — mild redness or swelling
• Headache — occasional, mild
• Nausea — rare and mild compared to PT-141 or Melanotan 2
• No bone density loss, hot flashes, or cardiovascular events reported in clinical trials
• Contraindicated in hormone-sensitive cancers (stimulates LH/FSH/sex steroids)
• Pregnancy — insufficient safety data; avoid
• PCOS caution — could worsen LH:FSH ratio imbalance

Frequently Asked Questions

What is the standard kisspeptin dose?

125 mcg subcutaneous daily, 1 hour before bed, for 30 days on / 30 days off. Draw 5 units from a 5 mg vial reconstituted with 2 mL BAC water.

What is the difference between kisspeptin-10 and kisspeptin-54?

Kisspeptin-54 is the full-length form with ~28 minute half-life and 4-6 hours of LH elevation. Kisspeptin-10 is the minimum active fragment with ~4 minute half-life. Different dose ranges and frequencies needed.

Why is pulsatile dosing important?

Continuous kisspeptin exposure desensitizes KISS1R within hours, suppressing LH/FSH. Discrete daily injections mimic natural pulsatile signaling and maintain receptor sensitivity.

How long should a kisspeptin cycle last?

30 days on, 30 days off per the standard protocol. This prevents receptor desensitization while providing sustained stimulation.

Can kisspeptin be used with PT-141?

They work through completely different mechanisms (GnRH pathway vs MC4R). Some researchers combine them for complementary effects, but no clinical data exists on the combination.

How do I reconstitute kisspeptin?

Add 2 mL BAC water to a 5 mg vial (2,500 mcg/mL). 125 mcg = 5 units on insulin syringe. Swirl gently, refrigerate, use within 28 days.

Related Guides

• Kisspeptin peptide page — vendor pricing and full profile
• Kisspeptin Benefits & Research — mechanism, fertility applications, and research
• Kisspeptin vs PT-141 — hormonal regulation vs direct arousal comparison
• PT-141 Dosing Guide — FDA-approved melanocortin sexual health peptide
• Melanotan 2 Dosing Guide — related melanocortin peptide
• Calculate Your Kisspeptin Cycle Cost — estimate vials needed and total cost

References

For educational and research purposes only. This is not medical advice. Kisspeptin is not FDA-approved for any indication.