The clean number to remember: a 10 mg Pinealon vial reconstituted with 1 mL of bacteriostatic water gives 10 mg/mL, where a 5 mg draw is exactly 50 units on a 100-unit insulin syringe and 10 mg fills the syringe. Most reconstitution errors happen at the syringe-math step, not the mixing step.
Research-context information only. Pinealon is an unapproved research peptide with no FDA evaluation and no human clinical trials. This article reports how reconstitution math is documented in community sources, not what should be done. Consult a licensed physician for personal medical decisions.
This guide covers the supplies, the mixing process as community sources describe it, and the dilution charts for Pinealon. For dose and protocol context — including the fact that no trial dose exists — see the Pinealon dosing guide.
New to bacteriostatic water? Start with what it is, how much to use per vial, and how long it lasts — or see the full Bacteriostatic Water Guide.
What You Need
- A vial of lyophilized (freeze-dried) Pinealon — typically 10 mg
- Bacteriostatic water (0.9% benzyl alcohol)
- Insulin syringes (100-unit / U-100, 29-31 gauge)
- Alcohol swabs
- A clean, flat surface
Step-by-Step Reconstitution
The steps below describe how reconstitution is documented in community protocols and standard sterile practice — they report common practice, not a clinical instruction.
- Both vials are allowed to reach room temperature first. Cold peptide and cold water are documented to cause condensation.
- Both stoppers are swabbed — the Pinealon vial and the bacteriostatic water vial — with an alcohol swab, per standard sterile practice.
- The bacteriostatic water is drawn into the syringe. For the standard 10 mg/mL dilution, protocols describe drawing 1 mL (100 units) of bacteriostatic water.
- The water is added slowly down the side wall. Standard practice is to insert the needle at an angle so the water runs down the inside wall of the vial — not directly onto the powder.
- The vial is swirled, not shaken. Documented protocols describe swirling gently until the powder fully dissolves; shaking is reported to shear the peptide.
- The solution is inspected. A properly reconstituted solution is reported to be clear and free of particles.
Dilution Chart
10 mg vial + 1 mL bacteriostatic water = 10 mg/mL (the standard Pinealon dilution):
| Dose | Volume (mL) | Units (100-unit syringe) |
|---|---|---|
| 2.5 mg | 0.25 mL | 25 units |
| 5 mg | 0.50 mL | 50 units |
| 10 mg | 1.00 mL | 100 units |
10 mg vial + 2 mL bacteriostatic water = 5 mg/mL (more dilute — larger, easier-to-read draws):
| Dose | Volume (mL) | Units (100-unit syringe) |
|---|---|---|
| 2.5 mg | 0.50 mL | 50 units |
| 5 mg | 1.00 mL | 100 units |
Syringe Math
The conversion is deterministic. On a U-100 insulin syringe, 1 unit = 0.01 mL.
- Concentration = vial mg ÷ bacteriostatic water mL. For 10 mg + 1 mL: 10 mg/mL.
- Volume for a dose = dose mg ÷ concentration. For a 5 mg dose: 5 ÷ 10 = 0.5 mL.
- Units = volume mL × 100. For 0.5 mL: 50 units.
