The clean number to start from: a 10 mg Thymalin vial plus 1 mL of bacteriostatic water gives 10 mg/mL. Thymalin is unusual among research peptides because the amounts documented for it are milligram-scale rather than microgram-scale, so a single 10 mg vial covers only one or two of the doses described in the historical literature — the dilution math is less about squeezing tiny draws out of a vial and more about matching a large, whole-vial-sized amount to an insulin syringe.
It is also a compound readers routinely mix up. Thymalin is a polypeptide fraction extracted from thymus tissue — a mixture of short peptides, not one defined sequence — which is a different thing from the two peptides it is most often confused with, and that difference changes every number below.
Research-context information only. Thymalin is a research peptide. Protocols, doses, and reactions reported below come from published research and self-reported community sources. This article reports what has been documented, not what should be done. There is no Thymalin-specific reconstitution or stability study; the preparation math here follows standard peptide-handling convention, the published clinical literature's saline/procaine intramuscular use, and community subcutaneous sources — not a validated protocol. Subcutaneous administration in particular has no controlled basis. Research-grade Thymalin is sold labeled for research use only — not for human consumption. Consult a licensed physician for personal medical decisions.
Distinguish it from two commonly-confused peptides. Thymalin (the thymus-extract fraction) is distinct from thymulin, a single zinc-dependent nonapeptide, and from thymosin alpha-1, a defined 28-amino-acid peptide. These are three different compounds, and reconstitution or concentration figures documented for one do not transfer to the others — a number pulled from a thymosin alpha-1 chart will not describe a Thymalin vial. This guide covers supplies, the mixing process, and dilution charts for Thymalin. For dose context, see the Thymalin dosing guide.
What You Need
- A vial of lyophilized (freeze-dried) Thymalin — Ion Peptide supplies it as a 10 mg vial
- Bacteriostatic water (0.9% benzyl alcohol) for the subcutaneous route community sources describe; the published clinical literature instead describes saline or procaine for intramuscular use
- Insulin syringes (100-unit / U-100, 29-31 gauge)
- Alcohol swabs
- A clean, flat surface
Step-by-Step Reconstitution
The steps below describe how reconstitution is documented in standard peptide-handling references and community resources. They are not instructions.
- Standard practice describes allowing both vials to reach room temperature before mixing. Cold peptide and cold liquid are documented to encourage condensation.
- Sterile practice describes swabbing both stoppers — the Thymalin vial and the water or saline vial — with an alcohol swab.
- Drawing the reconstitution liquid. For 10 mg/mL on a 10 mg vial, community protocols describe drawing 1 mL of bacteriostatic water; for 5 mg/mL, they describe 2 mL. The published clinical literature describes dissolving the vial in saline or procaine for intramuscular injection rather than bacteriostatic water.
- Community protocols describe adding the liquid slowly down the inside side wall — not aiming it directly onto the powder.
- Swirl, do not shake. Documented handling describes swirling gently until the fraction fully dissolves; shaking can shear peptides.
- A properly reconstituted solution is documented to be clear and free of particles. Because Thymalin is an extract rather than a single purified peptide, community sources note that minor cloudiness on first mixing is sometimes reported; unresolved particulate is treated as a reason to discard.
Dilution Chart
Anchored to the 10 mg vial Ion Peptide supplies. The published clinical literature describes 5-10 mg intramuscular doses in saline/procaine; community subcutaneous protocols describe similar milligram amounts reconstituted in bacteriostatic water. Both are shown as documented data points, not recommendations.
10 mg vial + 1 mL bacteriostatic water = 10 mg/mL (a full syringe is roughly the whole vial):
| Documented amount | Volume (mL) | Units (100-unit syringe) |
|---|---|---|
| 2.5 mg | 0.25 mL | 25 units |
| 5 mg | 0.50 mL | 50 units |
| 7.5 mg | 0.75 mL | 75 units |
| 10 mg | 1.00 mL | 100 units |
10 mg vial + 2 mL bacteriostatic water = 5 mg/mL (larger, easier-to-read draws for smaller amounts):
| Documented amount | Volume (mL) | Units (100-unit syringe) |
|---|---|---|
| 1 mg | 0.20 mL | 20 units |
| 2.5 mg | 0.50 mL | 50 units |
| 5 mg | 1.00 mL | 100 units |
| 10 mg | 2.00 mL | 200 units (two draws) |
Syringe Math
The conversion is deterministic. On a U-100 insulin syringe, 1 unit = 0.01 mL.
- Concentration = vial mg ÷ reconstitution mL. For 10 mg + 1 mL: 10 mg/mL.
- Volume for an amount = amount mg ÷ concentration. For a 5 mg amount at 10 mg/mL: 5 ÷ 10 = 0.5 mL.
- Units = volume mL × 100. For 0.5 mL: 50 units.
Why the scale is different here: because the amounts documented for Thymalin are measured in milligrams rather than micrograms, a single injection can approach a full insulin syringe. At 10 mg/mL a 10 mg amount is the entire 1 mL fill (100 units), so a 10 mg vial is close to one clinical daily dose from the historical literature rather than a multi-week supply. Diluting to 5 mg/mL spreads the same amount across a larger, easier-to-read draw but pushes a 10 mg amount past a single syringe.
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