DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nonapeptide first isolated from rabbit brain in 1977, named for its ability to promote slow-wave (delta) sleep. Research has since revealed a broader profile including stress-hormone modulation, neuroendocrine regulation, and potential applications in substance withdrawal. DSIP is not FDA-approved. This is not medical advice.
Research-context information only. DSIP is a research peptide. Protocols, doses, and reactions reported below come from published research and self-reported community sources. This article reports what has been documented, not what should be done. Consult a licensed physician for personal medical decisions.
DSIP Dosing Table
Match your vial size below — reconstitution and dose math update automatically.
| Dose | Syringe units | mL volume | Schedule |
|---|---|---|---|
| 100 mcg | 4 units | 0.04 mL | Pre-bed SubQStarter |
| 250 mcg | 10 units | 0.1 mL | Pre-bed SubQ (5-on/2-off)Standard |
| 500 mcg | 20 units | 0.2 mL | Pre-bed SubQAdvanced |
Math assumes U-100 insulin syringes (1 mL = 100 units). Verify your syringe matches before injecting. Round half-units to the nearest visible mark.
| Dose | Syringe units | mL volume | Schedule |
|---|---|---|---|
| 250 mcg | 5 units | 0.05 mL | Pre-bed SubQ (5-on/2-off)Standard |
| 500 mcg | 10 units | 0.1 mL | Pre-bed SubQ |
Math assumes U-100 insulin syringes (1 mL = 100 units). Verify your syringe matches before injecting. Round half-units to the nearest visible mark.
Quick Reference: Standard Protocol
| Parameter | Standard Protocol |
|---|---|
| Dose | 250 mcg |
| Route | Subcutaneous injection |
| Timing | 1-3 hours before bed |
| Frequency | 5 days on, 2 days off |
| Cycle | 8 weeks on, 8 weeks off |
| Vial size | 5 mg |
| Reconstitution | 2 mL bacteriostatic water → 2,500 mcg/mL |
| Draw amount | 10 units on insulin syringe |
| Storage | Refrigerate 2-8°C, use within 28 days |
Standard protocol: 250 mcg subcutaneous, 1-3 hours before bed, 5 days on / 2 days off, for 8-week cycles.
Cycling: 8 Weeks On, 8 Weeks Off
Community protocols describe 250 mcg nightly (5 on / 2 off) for 8 weeks, then 8 weeks off. Schneider-Helmert's trials noted that sleep improvements persisted beyond the active dosing window, suggesting the off-cycle period often continues to provide benefit.
While no clinical evidence demonstrates DSIP tolerance development, cycling maintains receptor sensitivity and allows residual effects to be monitored during the off period.
Enhanced Protocol (Community)
Some practitioners reference alternative cycling patterns:
Dr. Seeds protocol: 100 mcg subcutaneously at night, administered daily, every 3 days, or weekly depending on individual response.
Pulse protocol: 100-200 mcg nightly for 10 consecutive days, followed by 20 days off. This leverages DSIP's delayed effects where improvements persist beyond active dosing.
The 250 mcg / 5-on / 2-off pattern is the most commonly documented community starting protocol. The alternatives above reflect additional community variations.
Routes of Administration
Subcutaneous injection (standard): Protocols describe injecting into the abdominal area (2+ inches from navel), thigh, or upper arm using a 29-31 gauge insulin syringe, rotating injection sites.
Clinical context: Early studies used IV infusions at 25 nmol/kg with morning timing and still observed delayed sleep benefits, but evening SC injection is the community standard.
Reconstitution Quick Reference
| Vial Size | BAC Water | Concentration | 250 mcg Dose |
|---|---|---|---|
| 5 mg | 2 mL | 2,500 mcg/mL | 10 units |
Math: 5,000 mcg / 2 mL = 2,500 mcg/mL. For 250 mcg: 250 / 2,500 = 0.1 mL = 10 units. One vial lasts 20 doses.
Community protocols describe swirling gently rather than shaking, refrigerating at 2-8°C, and using within 28 days. For step-by-step instructions, see the Reconstitution Calculator.
