How to Reconstitute CJC-1295: Mixing & Dosing Guide (2026)
CJC-1295 reconstitution guide: 2mL BAC water into a 2mg vial = 10 units per 100mcg dose. Dilution charts, timing, and storage.
How to Reconstitute CJC-1295 (No DAC)
CJC-1295 without DAC — also known as Mod GRF (1-29) — is a modified growth hormone-releasing hormone analog. It tells your pituitary to release growth hormone, but unlike the DAC version, it works in short bursts that mimic your body's natural pulsatile GH secretion.
The reconstitution process is standard peptide preparation. But because CJC-1295 no DAC has a short half-life (roughly 30 minutes), timing and dose precision matter more than with longer-acting compounds.
What You Need
Before you start, gather everything:
- CJC-1295 (no DAC) lyophilized vial (typically 2mg or 5mg)
- Bacteriostatic water (BAC water) — not sterile water, not saline
- Insulin syringes — 1mL (100 unit), 29-31 gauge
- Alcohol swabs — for cleaning vial tops
- A clean, flat workspace
Why bacteriostatic water? It contains 0.9% benzyl alcohol that prevents bacteria from growing in your solution. Regular sterile water has no preservative, so the vial must be used within 24 hours. BAC water gives you up to 28 days refrigerated.
Step-by-Step Reconstitution
Step 1: Clean Everything
Wipe the tops of both vials (CJC-1295 and BAC water) with alcohol swabs. Let them air dry for 10 seconds. This prevents contamination — skip it at your own risk.
Step 2: Draw Your Bacteriostatic Water
Using a fresh insulin syringe, draw your desired amount of BAC water. The amount you add determines your concentration (see the dilution charts below).
For a 2mg vial, 2mL gives you the cleanest math. 100mcg doses come out to exactly 10 units.
Step 3: Add Water to the Peptide Vial
Insert the needle into the CJC-1295 vial at an angle, aiming at the glass wall — not directly at the powder. Let the water trickle down the side of the vial gently.
Do not squirt water directly onto the powder. Peptides are fragile. Aggressive mixing can damage the molecular structure and reduce potency.
Step 4: Let It Dissolve
Gently swirl the vial with a slow rotating motion. Do not shake it. The powder should dissolve within 1-2 minutes into a perfectly clear, colorless solution.
If particles remain after 5 minutes of gentle swirling, the peptide may be degraded. A properly manufactured CJC-1295 dissolves easily.
Step 5: Store Correctly
Refrigerate immediately at 36-46F (2-8C). The reconstituted solution is stable for up to 28 days with bacteriostatic water.
Dilution Charts
2mg Vial
| BAC Water Added | Concentration | 100mcg Dose | 150mcg Dose |
|---|---|---|---|
| 1mL | 2,000mcg/mL | 5 units (0.05mL) | 7.5 units (0.075mL) |
| 2mLRecommended | 1,000mcg/mL | 10 units (0.1mL) | 15 units (0.15mL) |
5mg Vial
| BAC Water Added | Concentration | 100mcg Dose | 150mcg Dose |
|---|---|---|---|
| 1mL | 5,000mcg/mL | 2 units (0.02mL) | 3 units (0.03mL) |
| 2mLRecommended | 2,500mcg/mL | 4 units (0.04mL) | 6 units (0.06mL) |
| 2.5mL | 2,000mcg/mL | 5 units (0.05mL) | 7.5 units (0.075mL) |
Why 2mL? It gives you clean, round numbers for standard doses — easy to measure, hard to mess up.
The Math (So You Can Do It Yourself)
Here's the formula for any vial size and any amount of water:
Concentration = Peptide Amount (mcg) / Water Added (mL)
Then to find your injection volume:
Units to inject = Desired Dose (mcg) / Concentration (mcg/mL) x 100
Example: 2,000mcg vial + 2mL water = 1,000mcg/mL. For a 100mcg dose: 100 / 1,000 x 100 = 10 units.
Don't want to do math? Use our Reconstitution Calculator — plug in your vial size, water amount, and desired dose, and it does the rest.
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Common Mistakes
Shaking the Vial
Peptides are proteins. Shaking creates foam, which means air bubbles trapped against peptide molecules. This can denature (destroy) the peptide at the air-liquid interface. Always swirl gently.
Using Too Little Water
Adding 0.5mL to a 2mg vial gives you 4,000mcg/mL. A 100mcg dose is only 2.5 units — nearly impossible to measure accurately on an insulin syringe. Use at least 1mL, ideally 2mL.
Leaving It at Room Temperature
Reconstituted CJC-1295 degrades at room temperature. Pull the vial out, draw your dose, put it back. The short half-life of the active peptide in your body is separate from solution stability, but degradation in the vial is still a concern.
Reusing Needles
Each puncture through the rubber stopper dulls the needle and increases contamination risk. Fresh syringe every time — they cost pennies.
Freezing Reconstituted Peptide
Freezing a liquid peptide solution creates ice crystals that can shear the peptide bonds apart. Only freeze lyophilized (powder) peptides. Once reconstituted, refrigerate — never freeze.
Ignoring Timing
This is the most common mistake specific to CJC-1295 no DAC. With a 30-minute half-life, when you inject matters. Inject on an empty stomach — food (especially carbs and fats) blunts the GH pulse. Most protocols time injections before bed, upon waking, or post-workout. Eating within 30 minutes of injection significantly reduces effectiveness.
How Many Doses Per Vial?
This depends on your dose and vial size:
2mg vial at 100mcg/dose = 20 doses 2mg vial at 150mcg/dose = 13 doses 5mg vial at 100mcg/dose = 50 doses 5mg vial at 150mcg/dose = 33 doses
At a standard protocol of 100mcg 1-3 times daily, a 2mg vial lasts 7-20 days. A 5mg vial lasts 17-50 days. Most users find the 5mg vial more economical.
Storage Quick Reference
| State | Temperature | Shelf Life |
|---|---|---|
| Lyophilized (powder) | Room temp | 6-12 months |
| Lyophilized (powder) | Refrigerated | 2+ years |
| Lyophilized (powder) | Frozen (-20C) | 3+ years |
| Reconstituted (BAC water) | Refrigerated | Up to 28 days |
| Reconstituted (sterile water) | Refrigerated | Use within 24 hours |
Pro tip: If you buy multiple vials, keep unopened ones in the freezer and only reconstitute what you'll use within 3-4 weeks.
Related Reading
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CJC-1295 Dosing Guide — protocols, timing, and cycles
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CJC-1295 Bloodwork Guide — what labs to track and optimal ranges
-
Ipamorelin Reconstitution Guide — the classic pairing partner
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BPC-157 Reconstitution Guide — healing peptide mixing guide
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GHK-Cu Reconstitution Guide — copper peptide for tissue repair
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BPC-157 Dosing Guide — commonly stacked healing peptide
References
- Teichman, S.L., et al. (2006). Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults. Journal of Clinical Endocrinology & Metabolism, 91(3), 799-805. PubMed:16352683
- Ionescu, M., et al. (2006). Pulsatile secretion of growth hormone (GH) persists during continuous stimulation by CJC-1295, a long-acting GH-releasing hormone analog. Journal of Clinical Endocrinology & Metabolism, 91(12), 4792-4797. PubMed:17018654
- Jette, L., et al. (2005). Human growth hormone-releasing factor (hGRF)1-29-albumin bioconjugates activate the GRF receptor on the anterior pituitary in rats: identification of CJC-1295 as a long-lasting GRF analog. Endocrinology, 146(7), 3052-3058. PubMed:15817669
- USP General Chapter 797: Pharmaceutical Compounding — Sterile Preparations. Storage and beyond-use dating guidelines for reconstituted peptides.
This guide is for educational and informational purposes only. It is not medical advice. CJC-1295 is sold as a research compound and is not FDA-approved for human use. Reconstituting and self-administering peptides carries inherent risks including infection, contamination, and dosing errors. Always use proper sterile technique. Consult a qualified healthcare provider before starting any peptide protocol. The Peptide Catalog is not responsible for any adverse effects resulting from the use or misuse of information presented here.