guidesApril 25, 2026·6 min read

Glow Blend Dosage Chart: 70mg 3-Peptide Protocol

10 units = 3.5mg: GHK-Cu 2.5mg + BPC-157 500mcg + TB-500 500mcg. Loading vs maintenance frequency, cycle length, and when Glow beats Klow.

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Glow Blend Dosing Guide

The Glow blend combines BPC-157 (10mg), TB-500 (10mg), and GHK-Cu (50mg) in a single 70mg vial — angiogenesis, cell migration, and collagen remodeling in one injection. It's the streamlined version of the healing peptide stack without the dedicated anti-inflammatory layer that the Klow blend provides.

Research-context information only. The GLOW blend is a research-peptide blend (containing GHK-Cu, BPC-157, and TB-500). Protocols, doses, and reactions reported below come from published research and self-reported community sources. This article reports what has been documented, not what should be done. Consult a licensed physician for personal medical decisions.

Research compounds without FDA approval. All protocols below are community-derived. This is not medical advice.

Glow Blend (GHK-Cu + BPC-157 + TB-500) Dosing Table

Match your vial size below — reconstitution and dose math update automatically.

Reconstitute: add 2 mL of bacteriostatic water to the 70 mg vial. Resulting concentration: 35 mg/mL.

Vials are labeled "50/10/10" — 50 mg GHK-Cu + 10 mg BPC-157 + 10 mg TB-500 (70 mg total).

1.75 mg blend5 units · 0.05 mL
Daily SubQ
Standard
3.5 mg blend10 units · 0.1 mL
Daily SubQ
Loading

Math assumes U-100 insulin syringes (1 mL = 100 units). Verify your syringe matches before injecting. Round half-units to the nearest visible mark.

Quick Reference: Community Protocol

Parameter Community Protocol
Dose 10 units on insulin syringe (3.5 mg total blend)
Route Subcutaneous injection
Timing AM
Loading frequency 5x/week (weeks 1-4)
Maintenance frequency 3x/week (weeks 5-12)
Cycle 8-12 weeks on, 4-8 weeks off
Vial size 70 mg
Reconstitution 2 mL bacteriostatic water → 35 mg/mL
Storage Refrigerate, use within 28 days

Per 10-unit injection: GHK-Cu 2.50 mg, BPC-157 500 mcg, TB-500 500 mcg — all three within standard dosing ranges.

Peptide Amount in Vial Per 5 Units Per 10 Units
GHK-Cu 50 mg (71.4%) 1.25 mg 2.50 mg
BPC-157 10 mg (14.3%) 250 mcg 500 mcg
TB-500 10 mg (14.3%) 250 mcg 500 mcg

Cycling Details

Loading (Weeks 1-4): 10 units 5x/week (weekdays on, weekends off). Weekly totals: GHK-Cu 12.5 mg, BPC-157 2.5 mg, TB-500 2.5 mg. One vial lasts 4 weeks at this rate.

Maintenance (Weeks 5-12): 5-10 units 3x/week. One vial lasts ~6.5 weeks at maintenance. Total for a 12-week cycle: 2-3 vials.

Off period (4-8 weeks): Healing effects continue as tissue remodeling processes complete. Community protocols commonly include an assessment window before a subsequent cycle.

Reconstitution Quick Reference

Vial Size BAC Water Concentration 10-Unit Dose
70 mg 2 mL 35 mg/mL 3.5 mg total blend

70 mg vial + 2 mL BAC water = 35 mg/mL. A standard 10-unit dose delivers 3.5 mg total blend.

Community protocols describe gentle swirling — not shaking — to avoid degradation, with storage at 2–8°C and use within 28 days. For the full step-by-step walkthrough, see the Glow Reconstitution Guide.

Top Glow Blend Vendors

Ranked by price, COA availability, and reputation

1
Ascension PeptidesCOA
$125.00
2
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$88.00
3
Glacier AminosCOA
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Ready to buy? Compare verified vendors on our best Glow Blend sources page, or browse all coupon codes for up to 50% off.

Where These Numbers Come From

The Glow blend's dosing is based on combining established individual peptide protocols into a single injection with fixed ratios.

GHK-Cu (Pickart & Margolina, 2018): Activates 4,000+ genes involved in tissue remodeling, collagen synthesis, and stem cell recruitment. The 2.5 mg per injection falls within standard GHK-Cu research ranges.

BPC-157 (Sikiric et al., 2014): Drives angiogenesis through VEGF upregulation and nitric oxide modulation. The 500 mcg per injection matches the standard standalone BPC-157 dose.

TB-500 (Malinda et al., 1999): LKKTET actin-binding domain promotes cell migration and anti-fibrotic effects. The 500 mcg per injection aligns with standard TB-500 daily dosing.

The blend ratio heavily favors GHK-Cu (71.4%) because effective GHK-Cu doses are in the milligram range while BPC-157 and TB-500 work at microgram levels. At 10 units, all three peptides deliver within their standard ranges — the math works out cleanly.

Glow vs Klow vs Wolverine Stack

Feature Glow (70mg) Klow (80mg) Wolverine Stack (20mg)
BPC-157 10 mg 10 mg 10 mg
TB-500 10 mg 10 mg 10 mg
GHK-Cu 50 mg 50 mg --
KPV -- 10 mg --
Best for Repair + collagen/anti-aging Repair + inflammation Pure injury recovery

Commonly chosen for: Musculoskeletal repair, anti-aging, collagen, skin/hair goals without significant inflammation. Klow is commonly chosen for: Adding NF-kB anti-inflammatory coverage for systemic inflammation or gut issues. Wolverine is commonly chosen for: Pure injury recovery without the collagen/anti-aging layer.

Side Effects & Safety

  • Injection site redness — mild, resolves within an hour
  • Mild drowsiness — first week, especially from GHK-Cu
  • Temporary warmth at injection site
  • Nausea — typically first 2-3 doses only
  • Headache — may relate to BPC-157's nitric oxide effects
  • All three peptides are research compounds without FDA approval
  • No human clinical trials exist for this specific combination

mg to Units Conversion

On a standard 100-unit insulin syringe, each "unit" equals 0.01 mL (so 100 units = 1 mL). Because this is a pre-mixed blend, a single draw delivers all components together in fixed ratio. The GLOW blend is dosed by the volume drawn; the components stay in their fixed manufacturing ratio.

At the standard reconstitution from the Quick Reference above (70 mg vial + 2 mL BAC water, 35 mg/mL total), each draw delivers the following total blend dose.

Units (insulin syringe) Volume (mL) Total blend dose
5 units 0.05 mL 1.75 mg
10 units 0.1 mL 3.5 mg
15 units 0.15 mL 5.25 mg
20 units 0.2 mL 7 mg

These conversions reflect the dilutions documented in community reconstitution protocols. They report how the math is described, not a recommended dosing schedule.

Core Supplies for This Protocol

The essentials for running any reconstituted injectable: cold storage, accurate syringes, alcohol prep pads, and metabolic tracking.

Cooluli Classic 4L Mini Fridge
Storage

Cooluli Classic 4L Mini Fridge

Compact thermoelectric mini-fridge with heat/cool toggle. The most-mentioned dedicated peptide-storage fridge in research community sources — fits ~20 vials and runs quietly.

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BD Ultra-Fine 31G 0.3cc 5/16" Insulin Syringes (Box of 90)
Injection

BD Ultra-Fine 31G 0.3cc 5/16" Insulin Syringes (Box of 90)

0.3cc 31G syringes — each gradation marks 1 unit (vs 2 units on 1cc), making sub-50-unit peptide doses accurate. BD Ultra-Fine is the most widely-cited brand in peptide community sources.

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Med-Pride Alcohol Prep Pads — Medical-Grade, Individually Wrapped
Injection

Med-Pride Alcohol Prep Pads — Medical-Grade, Individually Wrapped

Medical-grade 70% isopropyl alcohol prep pads, individually wrapped and sterile single-use. Standard for cleaning vial stoppers and injection sites before sub-Q dosing.

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CONTOUR NEXT GEN Glucose Meter All-In-One Kit
Tracking

CONTOUR NEXT GEN Glucose Meter All-In-One Kit

Ascensia's CONTOUR NEXT GEN — the most clinically-validated home glucose meter. Includes 20 test strips + lancing device. Tracks the blood-sugar response GLP-1 users care about, especially during dose escalation.

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Frequently Asked Questions

What is the Glow blend?
Glow is a pre-mixed 3-peptide blend containing BPC-157 (10mg), TB-500 (10mg), and GHK-Cu (50mg) — 70mg total per vial. It combines tissue repair and anti-aging peptides into a single injection.
What doses do community protocols document for Glow?
Community-documented protocols describe 10 units on an insulin syringe (3.5 mg total blend: 2.5 mg GHK-Cu, 500 mcg BPC-157, 500 mcg TB-500) daily or 5x/week during loading, then 3x/week maintenance. Cycle 8-12 weeks on, 4-8 weeks off.
What does community protocol document for Glow reconstitution?
Community protocols document adding 2 mL of bacteriostatic water to the 70mg vial, yielding 35 mg/mL. Gentle swirling (not shaking) is the documented mixing method. Storage is at 2–8°C; community protocols note a 28-day use window after reconstitution.
What is the difference between Glow and Klow?
Klow adds KPV (10mg) to the Glow formula for NF-kB-mediated anti-inflammatory action. Glow is the tissue repair and collagen-focused blend; Klow adds systemic anti-inflammatory support.
How do users typically choose between Glow blend and individual peptides?
Blends are more convenient and cost-effective for standard protocols. Individual peptides give more control over exact doses and injection sites. For general recovery and anti-aging, Glow is simpler. For targeted injury treatment, individual peptides may be preferred.
What cycle lengths are documented in community protocols?
Standard cycles run 8-12 weeks, followed by 4-8 weeks off. Loading phase (5x/week) for 4 weeks, then maintenance (3x/week) for the remaining weeks.
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References

Citation Topic PMID
Pickart & Margolina, Int J Mol Sci (2018) GHK-Cu gene expression profiling, 4,000+ genes 29986520
Pickart, J Biomedicine & Biotechnology (2008) GHK-Cu collagen synthesis, copper pathways 19133135
Sikiric et al., Curr Pharm Des (2014) BPC-157 nitric oxide system interaction 23755725
Seiwerth et al. (2014) BPC-157 EGF receptor and wound healing 23782145
Chang et al., J Appl Physiol (2011) BPC-157 FAK-paxillin tendon fibroblast activation 21030672
Malinda et al., PNAS (1999) TB-500 LKKTET actin-binding cell migration 10469335
Bock-Marquette et al., Nature (2004) Thymosin Beta-4 cardiac protection 15565145
Sosne et al., Clin Ophthalmol (2010) Thymosin beta-4 wound healing and anti-inflammatory effects 19668473

For educational and research purposes only. This is not medical advice. The Glow blend contains research peptides with no FDA approval.