How to Reconstitute the Wolverine Stack
The Wolverine Stack is a pre-blended vial containing 10mg BPC-157 + 10mg TB-500 — 20mg total. Reconstitution follows the same process as any lyophilized peptide: add bacteriostatic water, swirl, refrigerate. The only difference is the math. Because you're dosing a blend, each injection delivers both peptides in a fixed 1:1 ratio without needing separate vials or syringes.
If you've reconstituted BPC-157 or TB-500 individually before, this will feel familiar. If this is your first peptide vial, follow every step carefully — most mistakes happen during mixing, not injection.
What You Need
Before you start, gather everything:
- Wolverine Stack vial (20mg lyophilized powder — 10mg BPC-157 + 10mg TB-500)
- Bacteriostatic water (BAC water) — not sterile water, not saline
- Insulin syringes — 1mL (100 unit), 29-31 gauge
- Alcohol swabs — for cleaning vial tops before every puncture
- Sharps container — for safe needle disposal
- A clean, flat workspace
Why bacteriostatic water? It contains 0.9% benzyl alcohol that prevents bacterial growth in your solution. Regular sterile water has no preservative — the vial must be used within 24 hours. BAC water gives you up to 28 days refrigerated, which matters when a single vial lasts 40 days at standard dosing (you'll reconstitute half the vial's worth of doses at a time, or use 2 vials per cycle).
Step-by-Step Reconstitution
Step 1: Clean Both Vial Tops
Wipe the rubber stoppers on both the Wolverine Stack vial and the BAC water vial with separate alcohol swabs. Let them air dry for 10 seconds. This prevents contamination — skip it at your own risk.
Step 2: Draw 2mL Bacteriostatic Water
Using a fresh insulin syringe (or a larger 3mL syringe for easier measurement), draw 2mL of BAC water. This is the recommended standard dilution for the 20mg Wolverine Stack vial.
2mL is the sweet spot. It produces clean, round numbers for every common dose — 5 units for 500mcg, 7.5 units for 750mcg. No mental math required.
Step 3: Add Water to the Vial — Slowly
Insert the needle into the Wolverine Stack vial at an angle. Aim the needle tip at the glass wall, not at the powder cake. Let the water trickle down the inside wall of the vial slowly.
Do not squirt water directly onto the powder. Both BPC-157 and TB-500 are fragile peptide chains. Aggressive force can shear molecular bonds and reduce potency. Let gravity do the work.
Step 4: Swirl Gently — Never Shake
Remove the syringe. Gently rotate the vial between your fingers with a slow swirling motion. The powder should dissolve within 1-3 minutes into a perfectly clear, colorless solution.
Never shake the vial. Shaking creates foam — air bubbles trapped against peptide molecules at the liquid-air interface. This can denature (permanently destroy) both peptides. If you see foam, you've been too aggressive.
If particles remain after 5 minutes of gentle swirling, the peptide may be degraded. Properly manufactured Wolverine Stack dissolves easily.
Step 5: Label and Refrigerate
Write the date and concentration (10mg/mL or 10,000mcg/mL) on the vial with a marker or label. Refrigerate immediately at 36-46F (2-8C). The clock starts now — use within 28 days.
Dilution Chart
20mg (10mg BPC-157 + 10mg TB-500) Vial
| BAC Water Added | Concentration | 500mcg Dose | 750mcg Dose |
|---|
| 1mL | 20,000mcg/mL | 2.5 units (0.025mL) | 3.75 units (0.0375mL) |
| 2mLRecommended | 10,000mcg/mL | 5 units (0.05mL) | 7.5 units (0.075mL) |
| 3mL | 6,667mcg/mL | 7.5 units (0.075mL) | 11.25 units (0.1125mL) |
Why 2mL? At 10,000mcg/mL, each unit on a U-100 insulin syringe equals exactly 100mcg of total blend. The standard 500mcg dose is exactly 5 units — no fractions, no guessing.
Syringe Math Explained
Here's the formula so you can verify any dilution yourself:
Concentration = Total Peptide (mcg) / Water Added (mL)
20,000mcg (20mg) / 2mL = 10,000mcg/mL
Units to inject = Desired Dose (mcg) / Concentration (mcg/mL) x 100
500mcg / 10,000mcg/mL x 100 = 5 units
On a U-100 insulin syringe, 1mL = 100 units. So each unit = 0.01mL. At our concentration, each unit delivers 100mcg of total blend.
| Dose |
Units (2mL recon) |
What You're Getting |
| 250mcg |
2.5 units |
125mcg BPC-157 + 125mcg TB-500 |
| 500mcg |
5 units |
250mcg BPC-157 + 250mcg TB-500 |
| 750mcg |
7.5 units |
375mcg BPC-157 + 375mcg TB-500 |
| 1,000mcg |
10 units |
500mcg BPC-157 + 500mcg TB-500 |
What's in Each Dose
This is where blends differ from single peptides. The Wolverine Stack is a 1:1 ratio — half BPC-157, half TB-500. Every dose you draw contains equal parts of both peptides.
- 500mcg dose = 250mcg BPC-157 + 250mcg TB-500
- 750mcg dose = 375mcg BPC-157 + 375mcg TB-500
- 1,000mcg dose = 500mcg BPC-157 + 500mcg TB-500
The 500mcg daily dose (250mcg per peptide) aligns with standard standalone dosing for both BPC-157 and TB-500. You don't need to supplement either peptide separately at this dose.
At 500mcg/day, one 20mg vial provides 40 doses — enough for 40 days. A standard 8-week (56-day) cycle requires 2 vials.
Storage Rules
| State |
Temperature |
Shelf Life |
| Lyophilized (powder) |
Room temperature |
6-12 months |
| Lyophilized (powder) |
Refrigerated (2-8C) |
2+ years |
| Lyophilized (powder) |
Frozen (-20C) |
3+ years |
| Reconstituted (BAC water) |
Refrigerated (2-8C) |
Up to 28 days |
| Reconstituted (sterile water) |
Refrigerated |
Use within 24 hours |
Key storage rules:
- Refrigerate immediately after reconstitution. Every hour at room temperature reduces potency.
- Never freeze reconstituted solution. Ice crystals shear peptide bonds apart. Only freeze lyophilized (powder) vials.
- Store upright. Keeps the rubber stopper from prolonged contact with the solution.
- Keep away from light. Direct sunlight accelerates degradation. The back of the fridge is ideal.
- Use a fresh syringe every draw. Each needle puncture through the stopper introduces contamination risk. Never reuse syringes.
Pro tip: If you buy multiple vials for an 8-week cycle, keep the second vial in the freezer as powder. Only reconstitute it when the first vial runs out (around day 28-30).
Common Mistakes
Spraying BAC Water Directly onto the Powder
The most common error. High-pressure water hitting the lyophilized cake can denature the peptide chains. Always aim at the glass wall and let the water roll down to the powder.
Shaking the Vial
Peptides are proteins. Shaking creates foam at the air-liquid interface, which denatures molecules at the boundary. If your solution is foamy, you've damaged some of the peptide. Always swirl gently.
Using Sterile Water Instead of BAC Water
Sterile water works for reconstitution, but it has no preservative. You must use the entire vial within 24 hours — impossible with 40 doses in a vial. BAC water's 0.9% benzyl alcohol keeps bacteria from growing for up to 28 days.
Storing at Room Temperature
Reconstituted peptides degrade rapidly above refrigerator temperature. Pull the vial out, draw your dose, put it back. Don't leave it on the counter while you prep your injection site.
Using the Same Syringe for Multiple Vials
Drawing from your BAC water vial and then inserting the same needle into your peptide vial transfers potential contaminants. Use a fresh needle for each vial when reconstituting. For daily draws from an already-reconstituted vial, a fresh insulin syringe each time is sufficient.
Wolverine Stack Blend vs. Mixing BPC-157 + TB-500 Separately
| Factor |
Pre-Mixed Blend |
Separate Vials |
| Convenience |
One vial, one reconstitution, one injection |
Two vials, two reconstitutions, two injections |
| Cost |
Typically cheaper per mg of total peptide |
More expensive — buying two separate products |
| Dose flexibility |
Fixed 1:1 ratio only |
Any ratio you want |
| Local injection |
Both peptides go to the same site |
BPC-157 near injury, TB-500 anywhere (it's systemic) |
| Storage |
One vial to track, one expiry date |
Two vials, two reconstitution dates |
| Oral BPC-157 |
Not possible — TB-500 isn't orally active |
Can take BPC-157 orally for gut protocols |
Choose the blend when: You're running a standard 1:1 protocol and want simplicity. One injection, one vial, one set of math.
Choose separate vials when: You need to inject BPC-157 locally near a specific injury, want different doses of each peptide, or plan to use oral BPC-157 for gut healing. See our BPC-157 Reconstitution Guide for individual vial mixing.
Frequently Asked Questions
How much bacteriostatic water do I add to a 20mg Wolverine Stack vial?
Adding 2mL of bacteriostatic water to a 20mg vial gives 10mg/mL concentration. For a 500mcg daily dose, that's 5 units (0.05mL) on an insulin syringe — very precise and easy to measure.
Is reconstituting a Wolverine Stack blend different from single peptides?
The process is identical — add BAC water slowly along the glass wall, swirl gently, never shake. The only difference is your dose contains both BPC-157 and TB-500 in fixed ratio, so you don't need separate vials or syringes.
How long does reconstituted Wolverine Stack last?
Stored at 2-8C (refrigerator) with bacteriostatic water, reconstituted Wolverine Stack is stable for up to 28 days. Never freeze reconstituted peptides and keep the vial upright.
Can I use a different BAC water volume for the Wolverine Stack?
Yes — 1mL gives higher concentration (20mg/mL, 2.5 units per 500mcg) and 3mL gives lower (6.67mg/mL, 7.5 units per 500mcg). Choose based on your syringe precision and how many doses you want per vial.
References
- Chang, C.H., et al. (2014). Pentadecapeptide BPC 157 enhances the growth hormone receptor expression in tendon fibroblasts. Molecules, 19(11), 19066-19077. PMC6271067
- Seiwerth, S., et al. (2018). BPC 157's effect on healing. Journal of Physiology-Paris, 112(1), 1-10.
- USP General Chapter 797: Pharmaceutical Compounding — Sterile Preparations. Storage and beyond-use dating guidelines for reconstituted peptides.
This guide is for educational and informational purposes only. It is not medical advice. The Wolverine Stack (BPC-157 + TB-500) is sold as a research compound and is not FDA-approved for human use. Reconstituting and self-administering peptides carries inherent risks including infection, contamination, and dosing errors. Always use proper sterile technique. Consult a qualified healthcare provider before starting any peptide protocol. The Peptide Catalog is not responsible for any adverse effects resulting from the use or misuse of information presented here.