The Glow blend is a 70mg vial containing three peptides: GHK-Cu (50mg), BPC-157 (10mg), and TB-500 (10mg). Reconstitution follows the same process as any lyophilized peptide — add bacteriostatic water, swirl, refrigerate. Each dose delivers all three peptides in a fixed ratio.
If you've mixed the Wolverine Stack or Klow blend before, this is identical. If it's your first time, follow every step carefully.
What You Need
Gather everything before starting:
- Glow blend vial (70mg lyophilized powder — 50mg GHK-Cu + 10mg BPC-157 + 10mg TB-500)
- Bacteriostatic water (BAC water) — not sterile water, not saline
- 3mL syringe with 18-25ga needle — for drawing and transferring BAC water
- Insulin syringes — 1mL (100 unit), 29-31 gauge, for daily injections
- Alcohol swabs — clean vial tops before every puncture
- Sharps container — safe needle disposal
- Clean, flat workspace
Why BAC water? It contains 0.9% benzyl alcohol that prevents bacterial growth. Sterile water has no preservative — use within 24 hours. BAC water gives you up to 28 days refrigerated, which matters when one Glow vial provides 20+ injections.
Step-by-Step Reconstitution
Step 1: Clean Both Vial Tops
Wipe the rubber stoppers on both the Glow vial and BAC water vial with separate alcohol swabs. Let them air dry for 10 seconds.
Step 2: Draw 2mL Bacteriostatic Water
Using the 3mL syringe, draw exactly 2mL of BAC water. This is the recommended dilution — it produces clean, round numbers for every common dose.
Step 3: Add Water to the Vial — Slowly
Insert the needle into the Glow vial at an angle. Aim the needle tip at the glass wall, not at the powder cake. Let the water trickle down the inside wall slowly.
Do not squirt water directly onto the powder. All three peptides are fragile chains. Aggressive force can shear molecular bonds and reduce potency.
Step 4: Swirl Gently — Never Shake
Remove the syringe. Gently rotate the vial between your fingers with a slow swirling motion. The powder should dissolve within 2-5 minutes into a clear solution. GHK-Cu may give a very slight blue-green tint — this is normal (copper complex).
Never shake. Shaking creates foam at the air-liquid interface, which can denature peptide molecules.
If particles remain after 5 minutes, refrigerate for 15-20 minutes and swirl again.
Step 5: Label and Refrigerate
Write the date and concentration (35mg/mL) on the vial. Refrigerate immediately at 36-46F (2-8C). Use within 28 days.
Dilution Chart
Why 2mL? At 35mg/mL total concentration, 10 units delivers exactly 3.5mg of total blend — clean, manageable math.
Syringe Math for All 3 Peptides
Concentration = Total Peptide (mg) / Water Added (mL)
70mg / 2mL = 35mg/mL
Units to inject = Desired Dose (mg) / Concentration (mg/mL) x 100
3.5mg / 35mg/mL x 100 = 10 units
Per-Peptide Breakdown by Dose
| Dose |
Units |
GHK-Cu |
BPC-157 |
TB-500 |
| 1.75mg |
5 units |
1.25mg |
250mcg |
250mcg |
| 3.5mg |
10 units |
2.50mg |
500mcg |
500mcg |
| 5.25mg |
15 units |
3.75mg |
750mcg |
750mcg |
The standard 10-unit dose (3.5mg total) delivers each peptide within its established standalone dosing range:
| Peptide |
Standard Solo Dose |
Glow @ 10 Units |
| GHK-Cu |
1-2mg/injection |
2.50mg |
| BPC-157 |
250-500mcg/injection |
500mcg |
| TB-500 |
250-750mcg/injection |
500mcg |
Vial Duration
At 10 units/injection, 5x/week (loading phase):
- 50 units per week
- 200 units per vial (2mL = 200 units on U-100 syringe)
- One vial lasts 4 weeks
At 10 units/injection, 3x/week (maintenance):
- 30 units per week
- One vial lasts ~6.5 weeks
Storage Rules
| State |
Temperature |
Shelf Life |
| Lyophilized (powder) |
Room temperature |
6-12 months |
| Lyophilized (powder) |
Refrigerated (2-8C) |
2+ years |
| Lyophilized (powder) |
Frozen (-20C) |
3+ years |
| Reconstituted (BAC water) |
Refrigerated (2-8C) |
Up to 28 days |
| Reconstituted (sterile water) |
Refrigerated |
Use within 24 hours |
Key rules:
- Refrigerate immediately after reconstitution
- Never freeze reconstituted solution — ice crystals shear peptide bonds
- Store upright — keeps stopper from prolonged contact with solution
- Keep away from light — sunlight accelerates degradation
- Fresh syringe every draw — each puncture introduces contamination risk
Pro tip: For multi-vial cycles, keep extras frozen as powder. Reconstitute only when the current vial runs out.
Common Mistakes
Spraying BAC Water Directly onto the Powder
High-pressure water hitting the lyophilized cake denatures peptide chains. Always aim at the glass wall and let water roll down to the powder.
Shaking the Vial
Peptides are proteins. Shaking creates foam that denatures molecules at the air-liquid boundary. If your solution is foamy, you've damaged some peptide. Always swirl gently.
Using Sterile Water Instead of BAC Water
Sterile water works but has no preservative. You'd need to use the entire vial within 24 hours — impossible with 20+ doses. BAC water's benzyl alcohol prevents bacterial growth for up to 28 days.
Storing at Room Temperature
Reconstituted peptides degrade rapidly above refrigerator temperature. Pull the vial out, draw your dose, put it back immediately.
Confusing Glow with Klow Vials
If you have both in your fridge, label clearly. Glow is 70mg (3 peptides), Klow is 80mg (4 peptides). The concentrations differ — drawing the wrong amount from the wrong vial will give incorrect dosing.
Frequently Asked Questions
How much bacteriostatic water do I add to a 70mg Glow blend vial?
Adding 2mL of bacteriostatic water to the 70mg vial gives 35mg/mL concentration. For a standard 3.5mg dose (10 units), each injection delivers 2.5mg GHK-Cu, 500mcg BPC-157, and 500mcg TB-500.
Is reconstituting a 3-peptide blend different from a single peptide?
The process is identical — add BAC water slowly along the glass wall, swirl gently, never shake. Each dose delivers three peptides in fixed ratio without needing separate vials or syringes.
How long does reconstituted Glow blend last?
Stored at 2-8C (refrigerator) with bacteriostatic water, reconstituted Glow is stable for up to 28 days. Never freeze reconstituted peptides and keep the vial upright.
What if my Glow solution has a slight blue-green tint?
Normal. GHK-Cu is a copper complex, and the copper ion can give the solution a very faint blue-green color. This is not degradation — it's expected chemistry.
Can I use a different BAC water volume?
Yes — 1mL gives higher concentration (70mg/mL, 5 units per 3.5mg) and 3mL gives lower (23.3mg/mL, 15 units per 3.5mg). 2mL is the sweet spot for clean syringe math.
References
- USP General Chapter 797: Pharmaceutical Compounding — Sterile Preparations. Storage and beyond-use dating guidelines for reconstituted peptides.
- Pickart, L., & Margolina, A. (2018). Regenerative and protective actions of GHK-Cu peptide. International Journal of Molecular Sciences, 19(7), 1987. PMID: 29986520
- Seiwerth, S., et al. (2018). BPC 157's effect on healing. Journal of Physiology-Paris, 112(1), 1-10.
This guide is for educational and informational purposes only. It is not medical advice. The Glow blend contains research peptides with no FDA approval. Reconstituting and self-administering peptides carries inherent risks including infection, contamination, and dosing errors. Always use proper sterile technique. Consult a qualified healthcare provider before starting any peptide protocol.