guidesApril 25, 2026·7 min read

Wolverine Stack Dosage Chart: 500mcg/Day

500mcg/day = 250mcg each, 5 units on an insulin syringe. BPC-157 finds the injury, TB-500 handles systemic repair. 8 weeks on, 2 vials.

Wolverine Stack Dosing Guide

The Wolverine Stack is the most popular healing peptide blend — BPC-157 (10mg) and TB-500 (10mg) pre-mixed in a single 20mg vial. BPC-157 drives localized repair (angiogenesis, growth factors), TB-500 handles the systemic side (cell migration, actin remodeling). For the full mechanism breakdown and synergy research, see the Wolverine Stack Benefits Guide.

Research-context information only. The Wolverine stack is a research-peptide blend (containing BPC-157 and TB-500). Protocols, doses, and reactions reported below come from published research and self-reported community sources. This article reports what has been documented, not what should be done. Consult a licensed physician for personal medical decisions.

Research compounds without FDA approval. All protocols below are community-derived. This is not medical advice.

Wolverine Stack (BPC-157 + TB-500) Dosing Table

Match your vial size below — reconstitution and dose math update automatically.

Reconstitute: add 2 mL of bacteriostatic water to the 20 mg vial. Resulting concentration: 10 mg/mL.

Vials are labeled "10/10" — 10 mg BPC-157 + 10 mg TB-500 (20 mg total). Doses split evenly between the two.

500 mcg blend5 units · 0.05 mL
Daily SubQ
Standard (250mcg of each)
1 mg blend10 units · 0.1 mL
Daily SubQ
Loading (500mcg of each)

Math assumes U-100 insulin syringes (1 mL = 100 units). Verify your syringe matches before injecting. Round half-units to the nearest visible mark.

Quick Reference: Community Protocol

Parameter Community Protocol
Dose 500 mcg total daily (250 mcg each peptide)
Route Subcutaneous injection
Timing AM, before food
Frequency Every day
Cycle 8 weeks on, 8 weeks off
Vial size 20 mg (10mg BPC-157 + 10mg TB-500)
Reconstitution 2 mL bacteriostatic water → 10 mg/mL
Draw amount 5 units on insulin syringe
Vials per cycle 2 (each lasts 40 days)
Storage Refrigerate, use within 28 days

Community-reported standard protocol: 500 mcg total daily (5 units) in the morning for 8 weeks. For localized injuries, community protocols describe injecting near the target area, providing local BPC-157 concentration alongside systemic TB-500 coverage.

Cycling Details

The standard cycle is 8 weeks on, 8 weeks off (or 4 weeks off minimum). Healing effects continue during the off period as tissue remodeling processes complete. Community sources commonly describe continued improvement for 2–4 weeks after stopping.

Loading protocol (aggressive): 750 mcg/day for 4 weeks, then 500 mcg/day for 8 weeks = 12 weeks total. For chronic injuries that haven't responded to standard dosing.

Twice-daily acute protocol: 500 mcg AM + 500 mcg PM (1,000 mcg total/day) for 4 weeks, then standard 500 mcg/day. For first 4 weeks post-surgery or acute injuries where speed matters. Community sources describe twice-daily dosing as beneficial for acute protocols given BPC-157's short half-life.

Routes of Administration

Subcutaneous (standard): For localized injuries, community protocols describe injecting near the target area. For systemic recovery, rotate between standard sites (abdomen, thigh, upper arm). TB-500 works systemically regardless of site; BPC-157 achieves higher local concentrations when injected nearby.

Injection sites: Lower abdomen (2 inches from navel), front of thigh, back of upper arm. Community injection protocols describe rotating sites to minimize lipodystrophy risk.

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Reconstitution Quick Reference

BAC Water Concentration 500 mcg Dose 750 mcg Dose Days per Vial
2 mL 10 mg/mL 5 units 7.5 units 40 days

20 mg vial + 2 mL BAC water = 10 mg/mL (10,000 mcg/mL). A 500 mcg dose is 5 units on an insulin syringe.

Community protocols describe swirling gently rather than shaking to preserve peptide integrity, with refrigeration at 2-8°C and use within 28 days. For the full step-by-step walkthrough, see the Wolverine Stack Reconstitution Guide.

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Where These Numbers Come From

The Wolverine Stack combines two well-established healing peptides at doses that align with their individual standalone protocols.

BPC-157 (Sikiric et al., 2018): Drives angiogenesis via VEGF, activates FAK-paxillin pathway in tendon fibroblasts (Chang et al., 2011), and upregulates GH receptor expression. The 250 mcg per-peptide dose is at the lower end of standalone BPC-157 protocols (250-500 mcg/day) — appropriate for a combination approach.

TB-500 (Malinda et al., 1999): LKKTET actin-binding domain promotes cell migration and anti-fibrotic tissue organization (Sosne et al., 2010). The 250 mcg per-peptide dose is below standalone TB-500 protocols (500 mcg/day) but provides complementary coverage alongside BPC-157.

Combination evidence (Lee & Padgett, 2021): A retrospective study compared BPC-157 alone to BPC-157 + Thymosin Beta-4 for knee pain. While limited (small sample, retrospective design), patients receiving the combination showed improved pain scores.

The synergy is straightforward: BPC-157 builds repair infrastructure (blood vessels, growth factors), TB-500 moves repair cells into position (migration, actin remodeling). One creates the environment; the other populates it.

Wolverine Stack vs Glow vs Klow

Feature Wolverine Stack (20mg) Glow (70mg) Klow (80mg)
BPC-157 10 mg 10 mg 10 mg
TB-500 10 mg 10 mg 10 mg
GHK-Cu -- 50 mg 50 mg
KPV -- -- 10 mg
Best for Pure injury recovery Repair + collagen/anti-aging Repair + inflammation
Typical price Lower Mid Higher

Wolverine Stack is most commonly chosen for pure injury recovery, tendon/joint healing, or post-surgical repair. Glow is the common choice for repair combined with collagen and anti-aging goals. Klow is commonly chosen when chronic inflammation is also a priority.

Stacking Protocols

Wolverine Stack + GHK-Cu

Peptide Dose Route Timing Purpose
Wolverine Stack 500 mcg daily SC AM Tissue repair + cell migration
GHK-Cu 1-3 mg 2x/week SC AM Collagen synthesis, gene activation

Or just buy the Glow blend which includes GHK-Cu pre-mixed.

Wolverine Stack + Ipamorelin

Peptide Dose Route Timing Purpose
Wolverine Stack 500 mcg daily SC AM Tissue repair
Ipamorelin 100-200 mcg SC Pre-bed GH release for enhanced recovery

BPC-157's GH receptor upregulation may synergize with increased GH output from Ipamorelin.

Side Effects & Safety

  • Injection site reactions — redness, minor swelling (resolves within hours)
  • Mild nausea — occasionally in the first few days at higher doses
  • Lightheadedness — uncommon, related to BPC-157's NO pathway activity
  • Fatigue — some report temporary tiredness in week 1 as repair ramps up
  • No human clinical trials for the BPC-157/TB-500 combination
  • Theoretical cancer concern — angiogenesis + cell migration peptides; no evidence of tumor promotion, but caution with active cancer
  • Purchase from COA-tested vendors — purity matters with research-grade compounds

mg to Units Conversion

On a standard 100-unit insulin syringe, each "unit" equals 0.01 mL (so 100 units = 1 mL). Because this is a pre-mixed blend, a single draw delivers all components together in fixed ratio. BPC-157 and TB-500 are present at the same concentration, so each draw delivers an equal dose of both.

At the standard reconstitution from the Quick Reference above (20 mg vial + 2 mL BAC water, 5,000 mcg/mL each), target doses convert as follows.

Dose (each peptide) Volume (mL) Units (insulin syringe)
125 mcg BPC-157 + 125 mcg TB-500 0.025 mL 2.5 units
250 mcg BPC-157 + 250 mcg TB-500 0.05 mL 5 units
375 mcg BPC-157 + 375 mcg TB-500 0.075 mL 7.5 units
500 mcg BPC-157 + 500 mcg TB-500 0.1 mL 10 units

These conversions reflect the dilutions documented in community reconstitution protocols. They report how the math is described, not a recommended dosing schedule.

Frequently Asked Questions

What is the Wolverine Stack?
The Wolverine Stack is a pre-mixed blend of BPC-157 (10mg) and TB-500 (10mg) in a single 20mg vial. It combines localized tissue repair (BPC-157) with systemic healing (TB-500) in one injection.
What doses do community protocols document for the Wolverine Stack?
Community-documented protocols report 500 mcg total daily (250 mcg BPC-157 + 250 mcg TB-500) via subcutaneous injection for 8 weeks. At the standard reconstitution (10mg/10mg vial + 2 mL BAC water = 10 mg/mL), this draws as 5 units on a U-100 insulin syringe.
How do community resources describe reconstituting the Wolverine Stack?
Community reconstitution resources describe adding 2 mL of bacteriostatic water to the 20mg vial, yielding 10 mg/mL. A 500 mcg dose draws as 5 units on a U-100 insulin syringe. Community sources report a 28-day use window when refrigerated at 2–8°C.
How long does one Wolverine Stack vial last?
A 20mg vial at 500 mcg/day lasts 40 days. At the standard 8-week (56-day) protocol, you need 2 vials per cycle.
Is the Wolverine Stack better than buying BPC-157 and TB-500 separately?
For standard 1:1 ratio protocols, the blend is more convenient, cheaper, and requires only one injection instead of two. If you need different doses of each peptide or want to inject BPC-157 locally near an injury, individual vials give more flexibility.
Do community sources describe injecting the Wolverine Stack near an injury?
Subcutaneous injection near the injury site is commonly described. TB-500 works systemically regardless of site, so a single injection provides both local BPC-157 concentration and systemic TB-500 coverage.

References

Citation Topic PMID
Sikiric et al., Curr Neuropharmacol (2018) BPC-157 brain-gut axis, pharmacological overview 29277310
Chang et al., J Appl Physiol (2011) BPC-157 tendon fibroblast activation, FAK-paxillin 21030672
Malinda et al., J Invest Dermatol (1999) TB-500 accelerates wound healing 10469335
Sosne et al., FASEB J (2010) TB-500 connective tissue repair, anti-fibrotic 20536458
Lee & Padgett, Alt Ther Health Med (2021) BPC-157 + TB-4 combination for knee pain 34324435
Seiwerth et al., Curr Pharm Des (2021) BPC-157 wound healing mechanisms PMC8275860

For educational and research purposes only. This is not medical advice. The Wolverine Stack contains research peptides with no FDA approval.