TB-500

thymosin beta-4 fragmentLyophilized powder

TB-500 is a synthetic peptide based on the active region of thymosin beta-4, a protein involved in cell migration and tissue repair. Its research comes mainly from animal and cell studies. Below: today's vial sizes, prices through our link and the vendor's COA details.

Size

$5.40/mg · In stock

Price

$27.00

List

$60.00

Buy 5 mg at Ascension Peptides

Price shown includes the 50% discount Ascension Peptides applies through our link.

Volume pricing at Ascension Peptides

3+ vials3% offin cart
5+ vials5% offin cart
10+ vials10% offin cart

Applied in Ascension Peptides's cart; how it combines with our discount is set at checkout.

Sold by Ascension Peptides. The Peptide Catalog does not sell peptides; we earn a commission if you buy through this link, at no extra cost to you. For laboratory research use only, not for human or veterinary use. Not FDA-approved. Nothing on this page is medical or dosing advice.

TB-500Ascension Peptides

Third-party testedResearch use only

Certificate of AnalysisVendor-stated: Third-party tested · HPLC · public COAView

Vendor-stated testing: Third-party tested · HPLC · public COA
Listed on our tb-500 vendor comparison, ordered by our vendor rankings
Lyophilized powder · laboratory research use only

Research-context information only. TB-500 is a research peptide. Protocols, doses, and reactions reported below come from published research and self-reported community sources. This article reports what has been documented, not what should be done. Consult a licensed physician for personal medical decisions.

Vial sizes listed by the vendor

TB-500 Vial Sizes & Prices

TB-500 vial sizes, prices and lots at Ascension Peptides
Size	List	Price	Per mg	Availability
5 mg	$60.00	$27.00	$5.40	In stock

Prices checked today, 5:03 AM CT on Ascension Peptides's site. Vendor-stated testing: Third-party tested · HPLC · public COA. The lot certificate is on Ascension Peptides's product page. Sizes not re-checked in the last 7 days are hidden. The vendor's checkout is final.

Test results

TB-500 Certificate of Analysis

Ascension Peptides publishes its certificates publicly (vendor-stated: Third-party tested · HPLC · public COA). Match the lot number on the vial to its certificate.

View the TB-500 certificate on Ascension Peptides's product page.

How to check one: how to verify a peptide COA.

TB-500 Dosing in Clinical Research

Healing / Tissue Repair

Educational reference only. Individual responses vary. Consult healthcare provider before use.

Vial Size

10 mg

Reconstitution

2 ml BAC water

Dose

500 mcg (10 units on 1ml syringe)

Timing

Frequency

Every day

Duration

8 weeks on, 8 weeks off

Protocol Notes

Systemic effects — injection site doesn't matter. Often stacked with BPC-157 for synergy.

Why This Dosing Protocol

Why daily dosing? TB-500 benefits from consistent daily dosing to saturate tissues throughout the 8-week cycle (8 weeks on, 8 weeks off). This maximizes the healing response.

Why injection site doesn't matter?TB-500's small molecular weight and actin-binding mechanism allow systemic distribution. Subcutaneous injection anywhere is sufficient — no need to inject near the injury.

Full Dosing Guide Reconstitution Calculator Cycle Cost Calculator

Popular Stack Protocols1 stack

Commonly paired protocols from the peptide research community. Educational reference only.

BPC-157 + TB-500

Healing / Injury Recovery

BPC-157

Dose

500 mcg

Vial Size

10 mg

Reconstitution

2 ml BAC water

TB-500

Dose

500 mcg

Vial Size

10 mg

Reconstitution

2 ml BAC water

Timing

AM (can split AM/PM)

Frequency

Daily

Duration

8 weeks on, 8 weeks off

The gold standard healing stack. BPC-157 drives local angiogenesis and growth factor upregulation while TB-500 provides systemic tissue repair via actin sequestration and cell migration. Complementary, non-overlapping mechanisms. BPC-157 can be injected near injury site; TB-500 works systemically regardless of injection site.

How TB-500 Works

Healing Peptide — Thymosin Beta-4 Fragment

Target → Repair Signal → Tissue Healing → Outcomes

Target

G-Actin (Cytoskeletal Protein)

TB-500 is the active fragment of Thymosin Beta-4. It binds G-actin, a building block of the cell's structural framework. By regulating actin polymerization, TB-500 directly influences cell migration, blood vessel formation, and wound healing at the cellular level.

Cellular Signal

Actin Sequestration → Cell Migration + Repair

TB-500 sequesters G-actin monomers, promoting cell migration to injury sites and supporting new blood vessel growth. It also reduces inflammation through NF-kB pathway modulation and promotes stem cell maturation for tissue regeneration.

Systemic Effect

Widespread Tissue Repair + Anti-Inflammation

Unlike locally-acting peptides, TB-500 distributes systemically — injection site doesn't determine where healing occurs. It reduces inflammation body-wide, promotes tissue repair in muscles, tendons, ligaments, skin, and cardiac tissue.

What You Notice

Reduced Inflammation → Faster Healing → Flexibility

Reduced overall inflammation and stiffness within 1-2 weeks. Faster healing of injuries over weeks to months. Improved flexibility and range of motion. Often stacked with BPC-157 for complementary healing via different mechanisms.

What Makes This Peptide Different

TB-500's key advantage is systemic distribution— it doesn't matter where you inject it, the peptide travels throughout the body. This makes it ideal for multiple simultaneous injuries or general inflammation reduction. While BPC-157 works through growth factor pathways (best for targeted local healing), TB-500 works through actin regulation (best for systemic repair). The two use completely different mechanisms, which is why stacking them is so popular.

TB-500 Clinical Evidence

Two parallel grades per outcome — clinical RCT depth and real-world community adoption. See the Research Overview below for citations and detail.

Outcome	Clinical Evidence	Community Evidence
Soft tissue repair	Moderate	Moderate
Cardiac repair after MI	Preliminary	Weak
Wound healing and angiogenesis	Moderate	Moderate
Hair regrowth	Weak	Weak

TB-500 Research Overview

Outcome-by-outcome look at what the evidence actually supports for TB-500, including human vs. animal study counts and our editorial take on each.

Soft tissue and tendon repair

Clinical:ModerateCommunity:Moderate

Thymosin beta-4 promotes cell migration and angiogenesis in multiple injury models. The synthetic 'TB-500' fragment is assumed to behave similarly but has less direct study.

0 human studies · 15 animal studies

Key findings & citations

Increases endothelial cell migration and tubule formation in vitro.
Speeds dermal wound closure by roughly 40% in rodent full-thickness models.
Tendon fibroblast migration enhanced at low micromolar concentrations.

Top citations

Our take

Mechanistically credible. Most user reports describe weeks-to-months timelines for tendinopathies, not the day-scale claims you'll see on forums.

Cardiac repair after MI

Clinical:PreliminaryCommunity:Weak

Mouse infarct models show preserved ejection fraction and reduced scar after thymosin beta-4 administration. No human cardiac trial has finished.

0 human studies · 8 animal studies

Key findings & citations

Mouse LAD-ligation models show roughly 15% smaller infarct size vs. controls.
Hypothesized to reactivate epicardial progenitor cells.
TB4 was investigated as a regenerative cardiac therapy by RegeneRx — no completed Phase 3.

Top citations

Bock-Marquette 2004 — Mouse MI model

Our take

Interesting science, not a clinical option. Unrelated to typical user goals around tendon and joint repair.

Hair regrowth

Clinical:WeakCommunity:Weak

A single mouse study showed accelerated hair follicle re-entry into anagen after TB4 treatment. Human evidence is essentially absent.

0 human studies · 1 animal study

Key findings & citations

Mouse follicle anagen entry shortened vs. saline controls in one published model.
No controlled human study — anecdotal reports only.
Mechanism is plausible (stem-cell migration) but unproven for hair specifically.

Top citations

Philp 2004 — Mouse hair follicle stem cells

Our take

Don't pick TB-500 for hair. Better-studied options exist (minoxidil, finasteride, GHK-Cu topically).

What TB-500 doesn't do

Claims that current evidence does not support. Worth knowing before you set expectations.

Acute injury repair within days
Healing timelines in published animal models are weeks-to-months. Reports of dramatic next-day improvement are inconsistent with the underlying biology.
Immediate post-surgery recovery
No human surgical-recovery trial has been completed. Anecdote-only territory, and prudent surgeons want clean inflammation control in the first two weeks.
Muscle hypertrophy or strength gains
TB4's mechanism is repair and migration, not anabolism. There is no evidence it increases muscle protein synthesis beyond baseline.

Technical specifications

TB-500 chemical & analytical data

Also known as: thymosin beta-4 fragment
Receptor targets: Actin-sequestering (thymosin beta-4 fragment)
Backbone length: 7 amino acids
Modifications: Synthetic fragment of thymosin beta-4 active site
Half-life (clinical PK): ~2–3 hours
Form: Lyophilized (freeze-dried) powder in a sealed vial
Presentation: 5 mg
Regulatory status: Research peptide; research-grade vials are not FDA-approved.

TB-500 Reconstitution Calculator

Dilution math and unit conversions. Prefilled using a common vial size for this peptide.

Open calculator

Storage & Handling

Storage at a glance

Proper storage temperatures, shelf life, reconstitution best practices, and travel tips for lyophilized and reconstituted peptides.

Powder: Freezer

1+ year at -20°C

Reconstituted: Fridge

4 weeks max at 2-8°C

View Complete Storage Guide

Handling specifics

Educational overview on storage, labeling, and traceability considerations for lab environments. Consult primary literature and vendor documentation for specifics.

Powder storage (very stable)

Freezer (-20°C): 1+ year
Refrigerator (2-8°C): 1-3 months
Room temperature: 2-3 weeks (emergency only)

Reconstituted storage (fragile)

MUST refrigerate at 2-8°C
4-week maximum shelf life
NEVER freeze after reconstitution
Use bacteriostatic water for multi-dose

11 answers

TB-500 FAQ: Buying, COA, Storage & Research

What is TB-500 and how does it differ from BPC-157?

TB-500 is a synthetic fragment of Thymosin Beta-4, a naturally occurring peptide involved in tissue repair. While both promote healing, TB-500 works more systemically (throughout the body) and focuses on cell migration and blood vessel formation, while BPC-157 tends to work more locally at injury sites.

What is TB-500 researched for?

TB-500 is studied for wound healing, tissue repair, flexibility, reducing inflammation, and promoting hair growth. It's popular in research for muscle injuries, tendon repair, and overall recovery from tissue damage.

What's the difference between TB-500 and Thymosin Beta-4?

TB-500 is a synthetic fragment designed to mimic the active region of the full-length Thymosin Beta-4 (TB-4) peptide. TB-4 is the complete 43-amino acid peptide with full biological activity across multiple pathways, while TB-500 is a simplified, more affordable version. TB-4 is considered more potent but costs more.

How does TB-500 work?

TB-500 promotes healing through several mechanisms: upregulating actin (a protein crucial for cell structure and movement), promoting cell migration to injury sites, stimulating angiogenesis (new blood vessel formation), and reducing inflammation. It helps cells move to where they're needed for repair.

What is the typical TB-500 dosage?

A common research dosage is 500 mcg per injection (10 units on 1ml syringe) administered every day in the AM. Protocol: 8 weeks on, 8 weeks off. Injection site doesn't matter as TB-500 has systemic effects throughout the body.

Can TB-500 help with flexibility?

Yes, TB-500 is noted for potentially improving flexibility and reducing muscle stiffness. This may be due to its effects on tissue remodeling and reducing adhesions. Many users report improved range of motion and reduced muscle tightness, especially when recovering from injuries.

Is TB-500 good for hair growth?

Research suggests TB-500 may promote hair growth by stimulating hair follicle stem cells and improving blood flow to the scalp. Some users report thicker, faster-growing hair. It's sometimes combined with other hair-supporting peptides like GHK-Cu for enhanced effects.

What are the side effects of TB-500?

TB-500 is generally well-tolerated. Possible side effects include temporary lethargy, head rush, and injection site reactions. Some report mild flu-like symptoms initially. Like most healing peptides, there are theoretical concerns about promoting growth in existing tumors, though not demonstrated in research.

How long does reconstituted peptide last?

Once mixed with bacteriostatic water, peptides remain stable for up to 4 weeks when refrigerated at 2-8°C (36-46°F). Unopened powder can last 1+ year in the freezer. Get our complete Storage & Travel Guide.

Is this peptide legal to purchase?

Peptides sold "for research purposes only" are legal to purchase in the US, but are not FDA-approved for human use outside of specific medical applications. Always consult a healthcare provider before use.

New to peptides?

Take our Peptide Match tool to find the best peptide for your goals. You can also read our Complete Guide to Peptides to learn the basics.

TB-500 Studies & Sources

The following peer-reviewed studies and official resources provide additional scientific context for this peptide:

TB-500 Research Articles

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TB-500

TB-500 Vial Sizes & Prices

TB-500 Certificate of Analysis